HC Agar Base

Formula(g/L):
Tryptone                     2.5
Proteose peptone         2.5
Yeast extract powder    5.0
Glucose                     20.0
Disodium hydrogen phosphate        3.5
Potassium dihydrogen phosphate      3.4
Ammonium chloride        1.4
Magnesium sulfate        0.06
Chloramphenicol            0.1
Sodium carbonate          1.0
Agar                           15.0
Final pH7.0±0.2 at 25℃

Directions:

Suspend 32.0g of the medium and 20ml of tween 80 in 1000ml of distilled water .Mix well and heat with frequent agitation. Dispense into tubes or triangle flasks. Sterilize in the autoclave at 121℃(15 lbs.) for 15 minutes.