Home-> Characteristic Product Series-> Granular Medium-> Soybean-Casein Digest Agar Granule

Soybean-Casein Digest Agar Granule

Chinese Name:	胰蛋白胨大豆琼脂颗粒
Product No.:	HBKP7026
Specification:	250g/500g
Product Price:
Product Use:	Used for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes.
Remarks:

Technical Data:

Approximate Formula:

Ingredients	gm/liter
Enzymatic Digest of Casein	15.0
Enzymatic Digest of Soybean Meal	5.0
Sodium Chloride	5.0
Agar	15.0
Final pH7.3±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend 40.0g of the medium in one liter of deionized or distilled water. Mix well and heat with frequent agitation. Boil for 1 minute. Dispense into tubes or flasks. Sterilize in the autoclave at121℃(15 lbs.) for 15 minutes.

Principle and Interpretation:

Soybean-Casein Digest Agar is used for the isolation and cultivation of nonfastidious and fastidious microorganisms.

The combination of casein and soy peptones in TSA renders the medium highly nutritious by supplying organic nitrogen, particularly amino acids and longer-chained peptides. The sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent.

Appearance:

Dehydrated medium is a free-flowing yellowish granule. The prepared medium is a kind of yellowish transparent gel.

Precautions:

This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

Soybean-Casein Digest Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Cultural characteristics observed after an incubation at 25±1℃ for 48 hours.

Microorganism	Strains Number	Inoculum (CFU)	Growth	Recovery	Remarks
Escherichia coli	ATCC 25922	20-100	luxuriant	≥70%	Colorless large colonies
Staphylococcus aureus	ATCC 6538	20-200	luxuriant	≥70%	Yellow colonies
Enterococcus faecalis	ATCC 29212	20-200	luxuriant	≥70%	White small colonies
Bacillus subtilis	ATCC 6633	20-200	luxuriant	≥70%	White irregular colonies
Pseudomonas aeruginosa	ATCC 9027	20-200	luxuriant	≥70%	Green pigment
Candida albicans	ATCC 10231	20-200	luxuriant	≥70%	White colonies (25℃,48-72h)
Aspergillus niger	ATCC 16404	20-200	luxuriant	≥70%	Black spore (25℃,48-72h)

Reference:

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. Japanese Ministry of Health, Labour and Welfare. 2006. The Japanese pharmacopoeia, 15th ed., online. Japanese Ministry of Health, Labour and Welfare.

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