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TCBS Agar Granule

Chinese Name:	TCBS琼脂颗粒
Product No.:	HBKD4130
Specification:	300mL/pack*10packs
Product Price:
Product Use:	Used for the selective isolation of Vibrios from a variety of clinical material, water and foods.
Remarks:

Technical Data:

Approximate Formula(gm/liter):

Yeast Extract	5.0	Sodium Chloride	10.0
Proteose Peptone	10.0	Ferric Ammonium Citrate	1.0
Sodium Thiosulfate	10.0	Bromothymol Blue	0.04
Sodium Citrate	10.0	Thymol Blue	0.04
Oxgall	8.0	Agar	15.0
Saccharose	20.0
Final pH 8.6±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend89.0 gof the medium in one liter of distilled water or deionized water. Heat with frequent agitation and boil for 1 minute. Cool to 45-50℃and pour in Petri dish. DO NOT AUTOCLAVE.

Principle and Interpretation:

TCBS Agar is highly selective for the isolation of Vibrios. Bile salts inhibits the growth of gram-positive bacteria. Sodium thiosulfate serves as a sulfur source and, in combination with ferric ammonium citrate, detects hydrogen sulfide production. Saccharose is included as a fermentable carbohydrate for the metabolism of Vibrios. The alkaline pH of the medium enhances the recovery of Vibrios cholerae. Thymol blue and bromthymol blue are included as indicators of pH changes.

Appearance:

Dehydrated medium is a free-flowing light grey granule.The prepared medium is a kind of blue-green gel.

Precautions:

This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

TCBS Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Inoculate and incubate at 36±1℃for 18-24 hours.

Microorganism	Strains Number	Inoculum (CFU）	Growth	Recovery	Remarks
Vibrio parahaemolyticus	ATCC 17802	20-200	Luxuriant	≥ 20%	Blue-green bamboo hat-like colonies, H₂S-
Vibrio alginolyticus	ATCC 33787	20-200	Luxuriant	≥ 20%	Yellow colonies, H₂S-
Escherichia coli	ATCC 25922	100-1000	Inhibited	< 1%	/
Staphylococcus aureus	ATCC 25923	100-1000	Inhibited	< 1%	/
Proteus vulgaris	ATCC 13315	100-1000	Poor/ Ihibited	< 10%	Center black and edge yellow colonies, H₂S +

Reference：

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. GB/T5750.12-2006 People's Republic of China Standard examination Methods for drinking water--Microbiological parameters.

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