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Simmons Citrate Agar

Chinese Name:西蒙氏枸橼酸盐琼脂
Product No.:HB0115
Specification:250g/500g
Product Price:
Product Use:Used for the differentiation of gram-negative bacteria on the basis of citrate utilization
Remarks:

Technical Data:

Simmons Citrate Agar is used for the differentiation of gram-negative bacteria on the basis of citrate utilization.

Approximate Formula:

Ingredients

gm/liter

Sodium Chloride

5.0

Magnesium Sulfate

0.2

Ammonium Dihydrogen Phosphate

1.0

Dipotassium Hydrogen Phosphate

1.0

Sodium Citrate

5.0

Bromothymol Blue

0.08

Agar

15.0

Final pH 6.8±0.2 at 25℃

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend 27.3g of the medium in one liter of deionized or distilled water. Mix well and heat with frequent agitation. Boil for 1 minute. Dispense into tubes. Sterilize in the autoclave at 121℃(15 lbs.) for 15 minutes.

Principle and Interpretation:

Organisms able to utilize ammonium dihydrogen phosphate and sodium citrate as the sole sources of nitrogen and carbon, respectively, will grow on this medium and produce an alkaline reaction as evidenced by a change in the color of the bromthymol blue indicator from green (neutral) to blue (alkaline). Bromothymol blue is pH indicator.

Appearance:

Dehydrated medium is a free-flowing light yellow-green powder.

The prepared medium is a kind of green transparent gel.

Precautions:

This medium is for laboratory use only.

Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

Simmons Citrate Agar must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Cultural characteristics observed after an incubation at 36±1℃for 24-48 hours.

Microorganism

Strains Number

Growth

Remarks

Escherichia coli

ATCC 25922

inhibited

Negative, green

Shigella flexneri

ATCC 12022

inhibited

Negative, green

Shigella sonnei

ATCC 25931

inhibited

Negative, green

Shigella dysenteriae

CMCC(B) 51252

inhibited

Negative, green

Salmonella typhimurium

ATCC 14028

good-luxuriant

Postive, blue

Citrobacter freundii

ATCC 43864

good-luxuriant

Postive, blue

Reference:

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. Simmons. 1926. J. Infect. Dis.39:209.

3. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, Md.

4. Holt, Krieg, Sneath, Staley and Williams (ed.). 1994. Bergey’s Manual™ of determinative bacteriology, 9th ed. Williams & Wilkins, Baltimore, Md.


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KF链球菌琼脂
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硝酸盐肉汤
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蜡样芽孢杆菌选择琼脂基础
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OGYE琼脂
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葡萄糖胰蛋白胨琼脂
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强化梭菌培养基(RCM)
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MRS琼脂
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MRS肉汤
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M17琼脂
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HB0391M17 Broth
M17肉汤
250g/500gUsed for isolating and enumerating lactic streptococci in yogurt, cheese starters and other dairy products
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沙氏葡萄糖肉汤培养基(USP)
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缓冲蛋白胨水BPW
250g/500gUsed for pre-enrichment of salmonella and Enterobacter sakazakii
HB4085Selenite Cystine Broth(SC)
亚硒酸盐胱氨酸增菌液(SC)
250g/500gUsed for salmonella selective enrichment culture (GB, SN standards)
HB4086-2Fluid Tetrathionate Medium
液体连四硫酸盐培养基
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HB4088Triple Sugar Iron Agar(TSI)
三糖铁(TSI)琼脂
250g/500gUsed for the differentiation of gram-negative enteric bacilli based on carbohydrate fermentation and the production of hydrogen sulfide.
HB2100-1Oxidase Strips
氧化酶试纸
10pcs Used for oxidase test
HB0101-10Plate Count Agar
平板计数琼脂
250g/500gUsed for the cultivation of bacteria and for the enumeration of microorganisms in water, sewage, faeces and other materials.
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煌绿乳糖胆盐肉汤(BGLB)
250g/500gUsed for the detection of coliform organisms in foods, dairy products, water and wastewater, as well as in other materials of sanitary importance.
HB0114-6Violet Red Bile Agar
结晶紫中性红胆盐琼脂(VRBA)
250g/500gUsed for the detection and enumeration of coliforms in milk, food and other materials.
HB0237-6Rose Bengal Agar
孟加拉红琼脂
250g/500gUsed for isolating and enumerating yeasts and molds.
HB4084-5Buffered Peptone Water
缓冲蛋白胨水
250g/500gUsed for preenriching injured Salmonella species from food specimens to increase recovery.
HB4085-5Selenite Cystine Broth
亚硒酸盐胱氨酸增菌液(SC)
250g/500gUsed as a selective enrichment medium for the isolation of Salmonella from feces, foods, water and other materials of sanitary importance.
HB4088-12Triple Sugar Iron Agar(TSI)
三糖铁(TSI)琼脂
250g/500gFor identification and the differentiation of Enterobacteria.
HB4090-3Bismuth Sulfite Agar
亚硫酸铋琼脂(BS)
250g/500gA highly selective medium used for isolating Salmonella spp., particularly Salmonella Typhi, from food and clinical specimens.
HB4094-1Lysine Decarboxylase Broth
赖氨酸脱羧酶培养基
250g/500gFor the identification of microorganisms, especially enteric bacilli,based on the decarboxylation of lysine.
HB4095-4Urea Agar Base
尿素琼脂(pH7.2)
250g/500gFor the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
HB4114Trypticase (Tryptic) Soy Broth
胰蛋白胨大豆肉汤
250g/500gA general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.
HB4115Baird-Parker Agar
Baird-Parker琼脂基础
250g/500gUsed for selective isolation and enumeration of coagulase-positive staphylococci
HB4118DNase Agar
DNA酶琼脂
100gDifferential media used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens
HB4123Staphylococcus Selective Agar
葡萄球菌选择性琼脂
250g/500gUsed for isolating and differentiating staphylococci based on mannitol fermentation and pigment formation
HB4128Mannitol Salt Agar
甘露醇高盐琼脂
250g/500gUsed for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials
HB4129Alkaline Peptone Water
碱性蛋白胨水
250g/500gFor selective enrichment culture of Vibrio cholerae (SN standards)
HB4130TCBS Agar
TCBS琼脂
250g/500gUsed for the selective isolation of Vibrios from a variety of clinical material, water and foods
HB4140HUGH LEIFSON Agar
O/F培养基(HLGB)
250g/500gUsed to distinguish between anaerobic and aerobic breakdown of carbohydrate (glucose)
HB4158Bromcresol Purple Broth Base
糖发酵基础肉汤
250g/500gUsed with added carbohydrate for differentiating microorganisms based on fermentation reactions.
HB4176-2Malt Extract Agar(MEA)
麦芽汁琼脂
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HB4188PALCAM Agar
PALCAM琼脂
250g/500gUsed with PALCAM Antimicrobic Supplement in isolating and cultivating Listeria, particularly from foods and milk products.
HB4193Fraser Medium
Fraser培养基
250g/500gEnrichment culture of Listeria
HB4198RVS Broth
RVS肉汤
250g/500gFor food inspection and selective enrichment of Salmonella (ISO standard)
HB4201-1Lysine Iron Agar
赖氨酸铁琼脂(LIA)
250g/500gUsed for the differentiation of enteric organisms based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulfide.
HB4105-8Xylose Lysine Deoxycholate Agar
木糖赖氨酸脱氧胆盐琼脂(XLD)
250g/500gA selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods.
HB4115-7Baird-Parker Agar Base
Baird-Parker 琼脂基础
250g/500gFor selective isolation and enumeration of coagulase-positive staphylococci.
HB4119-37.5% Sodium Chloride Broth
7.5% 氯化钠肉汤
250g/500gUsed for selective enrichment cultivation of Staphylococcus aureus.
HB8294-1Sodium Deoxycholate
去氧胆酸钠
100gUsed to inhibit the growth of Gram-positive bacteria.
HB8913Brilliant Green Tetrathionate Broth
四硫磺酸钠亮绿肉汤
250g/500gTetrathionate brilliant green broth with iodine-iodide Solution added, is a selective enrichment liquid medium used for the cultivation of salmonella typhi from feces,urine,foods and other materials.
JX1000335ONPG
ONPG
5gUsed for biochemical identification of salmonella and shigella
HB4096-140% Urea Water
40%尿素水
5mL*10 vialsEach vial was added to 95ml urease agar or 1000ml Urea- Egg Yolk double sugar Medium or 95ml MIU
HB0397TPY Agar Medium
TPY琼脂培养基
250g/500gUsed for the selective isolation of Bifidobacterium
HB4089Salmonella Shigella Agar (SS)
SS琼脂
250g/500gModerately selective and differential media for the isolation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella.
HB4090Bismuth Sulfite Agar
亚硫酸铋琼脂(BS)
250g/500gA highly selective medium used for isolating Salmonella spp., particularly Salmonella Typhi, from food and clinical specimens
HB4093Hektoen Enteric Agar(HE)
HE琼脂(HE)
250g/500gFor selective isolation (GB standard) Salmonella
HB4095-1Urea Agar Base
尿素琼脂(PH7.2)
250g/500gUsed for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
HB4095-2Christensen Urea Agar Base
柯氏尿素琼脂
250g/500gUsed for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
HB4095-3Urea Broth
尿素肉汤
250g/500gUsed as an aid in the identification of Enterobacteria, particularly to differentiate Proteus from Salmonella and Shigella from clinical samples.
HB4095Urea Agar Base
尿素酶琼脂基础
250g/500gUsed for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
HB4105XLD Agar
XLD培养基
250g/500gA selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods
HB4113Lactose Broth
乳糖肉汤
250g/500gUsed for detection of the presence of coliform organisms, as a pre-enrichment broth for salmonellae and in the study of lactose fermentation of bacteria in general.
HB4113-1Fluid Lactose Medium
液体乳糖培养基
250g/500gFor pre-enrichment culture of salmonella in food
HB4205BPLS Agar
BPLS琼脂
250g/500gUsed for the selective separation and cultivation of Salmonella in pathological materials, food, feces, etc.
HB5184-2Cetrimide Agar
溴化十六烷基三甲胺琼脂培养基(USP)
250g/500gUsed for the selective isolation and presumptive identification of Pseudomonas aeruginosa.
HB5190-4Fluid Thioglycollate Medium(USP)
硫乙醇酸盐流体培养基(USP)
250g/500gUsed for the sterility testing of biologics and for the cultivation of anaerobes, aerobes and microaerophiles.
HB5190Fluid Thioglycollate Medium(FTM)
液体硫乙醇酸盐培养基
250g/500gUsed for the sterility testing of biologics and for the cultivation of anaerobes, aerobes and microaerophiles
HB5215Fungal Agar
真菌琼脂培养基
250g/500gFor cultivation of fungi. Also used for carrying stock cultures of fungi and for chlamydospores production.
HB6231Mueller-Hinton Broth(MHB)
MH肉汤(MHB)
250g/500gUsed for the cultivation of microorganisms and for sensitivity testing.
HB6232MUELLER-HINTON Agar(MHA)
MH琼脂(MHA)
250g/500gFor antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the Clinical and Laboratory Standards Institute (CLSI).
HB6234Kligler Iron Agar
克氏双糖铁琼脂
250g/500gUsed for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to liberate sulfides.
HB6235Blood Agar Base
血液琼脂基础
250g/500gWith the addition of sterile blood, is used for the isolation, cultivation and detection of hemolytic activity of streptococci and other fastidious microorganisms.
HB6238MacConkey Agar
麦康凯琼脂
250g/500gUsed for the selective isolation and identification of colifroms and intestinal pathogens from feces, urine, wastewater and foods.
HB7026Soybean-Casein Digest Agar
胰蛋白胨大豆琼脂
250g/500gUsed for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes.
HB7031CLED Medium
CLED培养基
250g/500gFor the selective isolation and culture of microorganisms in urine
HB7040Yeast Nitrogen Base(YNB)
YNB培养基
100gUsed for classifying yeasts based on carbon assimilation.
HB8297-1Brain Heart Infusion Broth(BHI)
脑心浸液肉汤
250g/500gA highly nutritious infusion medium recommended for the cultivation of streptococci, Neisseria and other fastidious organisms.
HB8313MacConkey Broth(USP)
麦康凯肉汤(USP)
250g/500gEscherichia coli, coliform detection
HB8418Buffered Sodium Chloride Peptone Solution pH7.0
氯化钠-蛋白胨缓冲液
250g/500gUsed for pre-enrichment of bacteria or sample preparation.
HB8458MacConkey Agar(USP)
麦康凯琼脂培养基(USP)
250g/500gUsed for the selective isolation and identification of enterobacteria from feces, urine, wastewater and foods.
HB8469MS Medium
MS培养基
250g/500gBasic plant tissue culture medium
HB8478Brain Heart Infusion Agar
脑心浸液琼脂(BHI)
250g/500gA general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds.
HB8484-2Pseudomonas CN Agar
假单菌琼脂基础培养基/CN琼脂
250g/500gUsed for the selective isolation of Pseudomonas, especially for Pseudomonas aeruginosa.
HB8552Stuart Transport Medium
Stuart运送培养基
250g/500gUsed in the transport and preservation of specimens for the cultivation of diverse organisms such as gonococci, streptococci, enterobacteria, etc.
    
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