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Levine EMB is a weak selective medium, for isolation of intestinal bacteria, especially E. coli.
Approximate Formula
Components
Content(g/L)
Pancreatic digest of gelatin
10.0
Lactose
Dipotassium hydrogen phosphate
2.0
Agar
15.0
Eosin Y
0.4
Methylene Blue
0.065
Final pH 7.1±0.2 25℃
*Adjusted and/or supplemented as required to meet performance criteria.
Directions:
Suspend 37.4g of the medium in 1L of distilled water,sterilize in an autoclave for 15 min at 121℃.
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