Urine Orientation Chromogenic Medium (UTI) is a chromogenic medium for the presumptive
identification and differentiation of all the main micro-organisms that cause urinary tract infections
Formula:
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Ingredients
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gm/litre
|
|
Peptic digest of animal tissue
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15.0
|
|
Chromogenic mix
|
4.5
|
|
Agar
|
15.0
|
|
Final pH 7.0 ± 0.2 at 25℃
|
|
|
*Adjusted and/or
supplemented as required to meet performance criteria.
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Directions:
Suspend 34.5 grams of
Urine Orientation Chromogenic Medium (HB7013) in 1 litre of distilled
water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.
Description:
Urine Orientation Chromogenic Medium contains two specific chromogenic substrates which are cleaved by
enzymes produced byEnterococcus spp., E. coli and
coliforms. In addition, it contains tryptophan which indicates tryptophan
deaminase activity (TDA), indicating the presence of Proteus spp.
It is based on Cystine Lactose Electrolyte Deficient (CLED) Medium which
provides a valuable non-inhibitory diagnostic agar for plate culture of other
urinary organisms, whilst preventing the swarming of Proteus spp.
The chromogen, X-glucoside, is targeted towards ß-glucosidase enzyme activity,
and allows the specific detection of enterococci through the formation of blue
colonies.
The other chromogen, Red-Galactoside, is cleaved by the enzyme ß-galactosidase
which is produced by E. coli, resulting in pink colonies. Any
uncertainty in identification may be resolved by removing suspect colonies from
the plate and performing an indole test using Kovacs Indole Reagents (code HB8279).
The medium also contains tryptophan which acts as an indicator of tryptophan
deaminase activity (TDA), resulting in halos around the colonies of Proteus,
Morganella and Providencia spp.
Appearance:
Dehydrated Culture Medium
is a free-flowing straw -colored powder.
The prepared medium is a straw-colored, transparent agar.
Precautions:
Urine Orientation
Chromogenic Medium should only be used for in vitro diagnostic purposes.
Do not
use beyond the stated expiry date, or if the product shows any sign of
deterioration.
Storage conditions and Shelf life:
Urine Orientation
Chromogenic Medium must be stored tightly capped in the original container at
10-30°C.
The dehydrated medium
has a shelf life of 3 years from date of manufacture.
Prepared medium may be
stored, out of direct light for up to 2 weeks at 2-8°C.
Quality control:
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Test Organisms
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Incubation
|
Results
|
|
Time
|
Temperature
|
Atmosphere
|
|
Escherichia coli
ATCC® 25922
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth;
medium sized rose to magenta colonies
with darker pink centers
|
|
Klebsiella pneumoniae
ATCC® 13883
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth;
large, deep blue or dark indigo colonies
|
|
Enterococcus faecalis
ATCC® 29212
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth;
small, teal to turquoise colonies
|
|
Proteus mirabilis
ATCC® 12453
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; clear to light yellow colonies
with golden-orangecolor diffused
through surrounding media
|
|
Staphylococcus aureus
ATCC® 25923
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; opaque, cream to
white colored
colonies
|
|
Pseudomonas aeruginosa
ATCC® 27853
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; colorless to light yellow-green,
translucentcolonies, which may have
a slight
iridescence
|
|
Staphylococcus
saprophyticus
ATCC® 15305
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; opaque, pink colonies
|
|
Candida albicans
ATCC® 10231
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; small, white,moist colonies
|
|
Citrobacter freundii
ATCC® 8090
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth;
dark blue colonies, often with
a rose halo in the surrounding media
|
Reference:
1.Collee J. G.,
Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and cCartney, Practical
Medical Microbiology,1996, 14th Edition, Churchill Livingstone.
2.Pezzlo
M., 1998, Clin. Microbiol. Rev., 1:268-280.
3.Wilkie M. E.,
Almond M. K., Marsh F. P., 1992, British Medical Journal 305:1137-1141.
4.Friedman
M. P. et al, 1991, J. Clin. Microbiol., 29:2385-2389.
5.MurrayP., Traynor P.
Hopson D., 1992, J. Clin. Microbiol., 30:1600-1601.
6.Soriano
F., Ponte C., 1992, J. Clin. Microbiol., 30:3033-3034.
7.Merlino
et al, 1995, Abstr. Austr. Microbiol. 16(4):17-3.
Technical Data: