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Abstract£ºObjectiveTo study rapid propagation and in vitro flowering system for Dendrobium denndanum.MethodsStem with lateral buds of wild denndanum was used as explants and cultured on various culture media with different portions of hormone.ResultsThe best medium for lateral buds induction was the MS with addition of 6-BA (1.0 mg¡¤L-1),NAA (0.5mg/L), while MS with the addition of 6-BA (2.0 mg¡¤L-1),NAA (0.4 mg¡¤L-1) was suitable for the cluster shoots propagation. A few plantlets were induced flowering after cultured on MS medium with addition of 6-BA (2.0 mg¡¤L-1), the frequency of floral bud induction was 10.0%.ConclusionThe cluster shoots can be induced from the segments with lateral buds on the MS basic medium with addition of 6-BA and NAA.

Key words£ºDendrobium denndanum; Tissue culture; Induction of floral buds

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