志贺氏菌检验

一、培养基明细：
   名称                             规格                价格（元）
GN增菌液                            250g                 95
HE琼脂                              250g                 190
SS琼脂                              250g                 95
麦康凯琼脂                          250g                 85
伊红美蓝琼脂（EMB）                 250g                 85
三糖铁琼脂（TSI）                   250g                 85
葡萄糖半固体管                      20支                 20
半固体管                            20支                 20
葡萄糖铵琼脂                        250g                 90
尿素琼脂基础（pH7.2）               250g                 95
西蒙氏柠檬酸盐琼脂                  250g                 90
氰化钾生化鉴定管                    20支                 30
氰化钾生化鉴定对照管                20支                 20
赖氨酸脱羧酶肉汤                    20支                 25
氨基酸脱羧酶对照                    20支                 15
鸟氨酸脱羧酶对照                    20支                 20
鸟氨酸脱羧酶肉汤                    20支                 30
棉子糖发酵管                        20支                 30
甘露醇发酵管                        20支                 15
水杨苷发酵管                        20支                 40
七叶苷发酵管                        20支                 25
甘油生化管                          20支                 20
5%乳糖发酵管                        20支                 15
Kovacs靛基质试剂盒                 5ml*2                 20
蛋白胨水                            20支                 15
志贺氏菌属诊断血清                   *                    *
二、备注：
1、依据GB4789.5-2003 食品卫生微生物学检验 志贺氏菌检验，适用于食品和食物中毒样品中志贺氏菌的检验。
2、菌落特征：
   在HE琼脂平板或SS琼脂平板，麦康凯琼脂和伊红美蓝琼脂平板呈现无色透明不发酵乳糖的菌落。
3、在装有GN增菌液的广口瓶中培养志贺氏菌时，培养时间视细菌生长情况而定，当培养液出现轻微混浊时即应中止培养。   
   

 

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