Suspend 39g/liter,autoclave(15min at 121℃).Allow to cool to 45-50℃;add ,where necessary,gentamicin sulphate corresponding to 20mg of gentamicin base and pour into Petridishes. 称取本品39.0g,加热搅拌溶解于1000ml纯化水中,分装三角瓶121℃高压灭菌15分钟,冷至45-50℃左右时,如果需要可加入过滤除菌的硫酸庆大霉素20mg (每升培养基),混匀,倾入无菌平皿。